Explore Genetech-funded MPhil and MSc projects in Sri Lanka, focusing on Brucella detection, Aedes aegypti genetics, and coconut population structure. Uncover groundbreaking research and advancements! Research leading to the award of MPhil Determination of Population Structure of Yellow Dwarf Coconut Population in Sri Lanka Department of Botany. Chandima Kamral, M.Phil., Faculty of Applied Science, University of Sri Jayawardanapura; Coconut Research Institute, Lunuwila, Sri Lanka, 2015 (partially conducted at Genetech). Morphological and molecular identification of Oreochromis niloticus and its hybrids present in selected reservoirs in Sri Lanka. Saroopa Samaradivakara, M.Phil, Faculty of Veterinary Medicine and Animal Sciences, University of Peradeniya, Sri Lanka, 2009. Establishment of DNA based HLA Typing in Sri Lanka for forensic and transplant purposes. Induwara Gooneratne, M.Phil, Department of Forensic Medicine, Faculty of Medicine, University of Peradeniya, Sri Lanka, 2007. MSc Research Projects Detection and confirmed of Brucella abortus in bovine aborted fetuses by PCR in selected localities in Sri Lanka. Samudi N. K. Mohotti, M.Sc., Department of Microbiology, Faculty of Science, University of Kelaniya, Sri Lanka, 2017. Genetic characterization and molecular phylogeny of Aedes aegypti (Diptera: Culicidae) in Sri Lanka based on COI gene. H.A.K.M. Dharmarathne, M.Sc., IBMBB, University of Colombo, Sri Lanka, 2016. HLA Typing of MHC Class I Molecules by PCR & Sequencing in Sri Lanka. Rashmi Tippalagama, M.Sc., IBMBB, University of Colombo, Sri Lanka, 2012. Molecular epidemiology of Dengue virus. Barathy Logendra, M.Sc. in Experimental Biotechnology, Postgraduate Institute of Science University of Peradeniya, Sri Lanka 2010. Molecular Characterization of Chikungunya Virus isolates in Sri Lanka. Ishara Herath, M.Sc in Experimental Biotechnology, Postgraduate Institute of Science University of Peradeniya, Sri Lanka 2010. Molecular Characterization of human Cytomegalovirus in a cohort of immunocompromised patients in Sri Lanka. Imalka M. W. Gunasekera, M.Sc in Experimental Biotechnology, Postgraduate Institute of Science University of Peradeniya, Sri Lanka 2010. Suitability of using SSR markers to differentiate tissue cultured and other Musa varieties. Fintan. S. Gonsal, M.Sc. Postgraduate Institute of Agriculture, University of Peradeniya, Sri Lanka, 2009. Molecular characterization of Mycobacterium tuberculosis isolates in Sri Lanka utilizing the mycobacterium interspersed repetitive units (MIRU) typing method. Nandika Perera, M.Sc. Molecular Life Sciences, Institute of Biochemistry Molecular Biology and Biotechnology, University of Colombo, Sri Lanka, 2009. Development of a single step reverse transcriptase-polymerase chain reaction assay to detect chikungunya virus in clinical samples. Navodhi Wickremasinghe, M.Sc. in Experimental Biotechnology, Postgraduate Institute of Science University of Peradeniya, Sri Lanka 2009. Determination of sensitivity of multiplex and duplex PCR in the determination of Marek's disease virus. Shamini Akushla Wickramage, M.Sc in Experimental Biotechnology, Postgraduate Institute of Science University of Peradeniya, Sri Lanka 2009. Establishment of a Molecular Diagnostic System for Detecting Human Papillomavirus in Clinical Samples. Rubatharshini Ambikaibakan, M.Sc. in Experimental Biotechnology, Postgraduate Institute of Science University of Peradeniya, Sri Lanka 2008. Development of a low cost multiplex PCR-based diagnostic assay for Down Syndrome. A. Mary Renuka Fernando, M.Sc. in Experimental Biotechnology, Postgraduate Institute of Science University of Peradeniya, Sri Lanka 2008. Determination of the sensitivity of PCR based non-radiolabeled hybridization for the detection of Mycobacterium tuberculosis. K.A.D. Ravi Vishwajith, M.Sc. in Experimental Biotechnology, Postgraduate Institute of Science University of Peradeniya, Sri Lanka 2006. Establishment of a molecular diagnostic system for detecting Y-chromosomal microdeletions which cause male infertility. Thiruchelvam Kariharan, M.Sc. in Experimental Biotechnology, Postgraduate Institute of Science University of Peradeniya, Sri Lanka 2006.

Customizable course on molecular diagnostics of infectious diseases for professionals. Learn nucleic acid extraction, DNA probe development, PCR, and more. Molecular Diagnostics of Infectious Diseases This is a course which can be customised based on the request of degree awarding institutes, hospitals and groups of individuals such as lawyers, lecturers, scientists and teachers who wish to obtain a foundation in the theoretical and practical aspects of molecular diagnostics. Course Content: Molecular Diagnostic Tools Extraction of nucleic acids (DNA/RNA) from different clinical specimens Development of DNA probes and nucleic acid hybridization PCR (Polymerase Chain Reaction) PCR-Hybridization View FAQs Contact us If you are interested in doing this course at Genetech, please submit the Inquiry Form below or email us at info@genetechsrilanka.com to receive further details. View Inquiry Form

Genetech supports diverse student research in Sri Lanka, funding projects from PhD to BSc levels. Topics include forensic genetics, dengue vector studies, rice blast resistance, and chronic kidney disease. Student Projects Genetech has funded and facilitated student research projects for students from many universities in Sri Lanka. Anchor 1 Research leading to the award of PhD X-Chromosome markers for forensic genetics in Sri Lanka: development, validation and application of a novel 16 X-STR multiplex assay for the Sri Lankans. K. L. Nandika Perera, Ph.D., Department of Basic Sciences, Faculty of Health Sciences, The Open University of Sri Lanka. 2021 (Thesis Submitted). Population genetic and epidemiological investigation of the dengue vectors Aedes aegypti (L.) and Aedes albopictus (Skuse) in Sri Lanka. M. D. Nirmani, Ph.D., Department of Zoology and Environment Sciences, Faculty of Science, University of Colombo, Sri Lanka, 2021 (Thesis Submitted). DNA marker assisted breeding of rice blast resistant genes into local rice. Ama Jayawardana, Ph.D. Department of Plant Sciences, Faculty of Science, University of Colombo; Rice Research and development Institute, Batalagoda, 2019 (Conducted partially at Genetech). View more MPhil and MSc Research Projects Detection and confirmed of Brucella abortus in bovine aborted fetuses by PCR in selected localities in Sri Lanka. Samudi N. K. Mohotti, M.Sc., Department of Microbiology, Faculty of Science, University of Kelaniya, Sri Lanka, 2017. Genetic characterization and molecular phylogeny of Aedes aegypti (Diptera: Culicidae) in Sri Lanka based on COI gene. H.A.K.M. Dharmarathne, M.Sc., IBMBB, University of Colombo, Sri Lanka, 2016. Determination of Population Structure of Yellow Dwarf Coconut Population in Sri Lanka Department of Botany. Chandima Kamral, M.Phil., Faculty of Applied Science, University of Sri Jayawardanapura; Coconut Research Institute, Lunuwila, Sri Lanka, 2015 (partially conducted at Genetech). View more BSc Research Projects X-Chromosomal recombination – A family study analyzing 7 X-STR loci in Sinhalese. Ruvini Wijithalal, B.Sc. special in Zoology, Department of Zoology and Environment Science, University of Colombo, Sri Lanka, 2019. Detection of gene polymorphism in angiotensinogen protein among chronic kidney disease patients in Sri Lankan population. Sathiyamoorthy Kabaskar, B.Sc. (Hons) in Biomedical Science, School of Biomedical Sciences, British College of Applied Studies (BCAS Campus), 2018. Detection of gene polymorphisms in angiotensin II type 1 receptor among chronic kidney disease patients in Sri Lankan population. Shiyamnesh Ganesh, B.Sc. (Hons) in Biomedical Science, School of Biomedical Sciences, British College of Applied Studies (BCAS Campus), 2018. View more

Explore custom oligonucleotide and gene synthesis services with various modifications, purification options, free cloning, and sequencing for 100% accuracy. Oligonucleotide and Gene synthesis We offer primer synthesis services covering different concentration scales from 0.025 to 1µmole. Different purification options using techniques such as desalt, MOPC, HPLC and PAGE are available as per requirement of the customer. We also offer primer modifications such as fluorescent labelling, non-fluorescent modifications, dark quenchers and internal modifications. Oligonucleotide/Primer synthesis Gene synthesis We offer customised gene synthesis services in a plasmid DNA state with a free cloning service, as well as free DNA sequencing to confirm the sequence of the gene. Our customised gene synthesis service offers to generate genes based on the sequence requirement of the customer. With our free cloning service, the synthesised gene is made available in a plasmid DNA state. Our free sequencing service guarantees 100% sequence accuracy. Contact us

Explore diverse research on genetic variation, virological surveillance, and forensic DNA evidence in Sri Lanka, presented at various academic conferences and symposiums Conference Papers and Communications Page 1 of 2 Nirmani, M. D., Perera, K. L. N. S. & Galhena, G. H. (2020) A preliminary virological surveillance of field-caught adult Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) mosquitoes in selected sites in the District of Colombo and Gampaha of Sri Lanka. Proceedings of Annual Research Symposium 2020, University of Colombo; 44. De Mel, W. D. Y., Illeperuma, R. J., Fernando, S. P., Pusweldeniya, B. M. J. & Padmalal, U. K. G. K. (2019) Genetic variation of Elephas maximus maximus in Maduru Oya national park in Sri Lanka, age 2019: International Conference on Agriculture, Green Economics and Energy, Sapporo, Japan, 2019. Perera, N., Galhena, G. & Ranawaka, G. (2019) Development of a novel X-chromosomal STR based dacaplex PCR assay for the kinship analysis of Sinhalese population. Proceedings of the Open University Research Sessions (OURS 2019), Open University of Sri Lanka, P19. Available from: https://ours.ou.ac.lk/wp-content/uploads/2019/12/Abastracts-Open-University-Research-Sessions-October-2019-19-20.pdf Perera, N., Galhena, G. & Ranawaka, G. (2019) Genetic profiling of six X-chromosomal STR loci among Tamil populations in Sri Lanka. Proceedings of the 39th Annual Sessions of the Institute of Biology. Institute of Biology Sri Lanka, P36. Perera, N., Galhena, G. & Ranawaka, G. (2019) Haplotype analysis of two X-chromosome STR clusters in Sinhalese population. Proceedings of Annual Research Symposium, University of Colombo, P230. Nirmani, M. D., Perera, K. L. N. S. & Galhena, G. H. (2019) Suitability of six microsatellite markers for evolutionary genetic studies of Asian tiger mosquitoes in Sri Lanka. Proceedings of Annual Research Symposium. University of Colombo; 227. Nirmani, M. D., Perera, K. L. N. & Galhena, G. H. (2019) Microsatellite marker-based population dynamics of the dengue vector, Aedes albopictus in Wet and Dry Zones of Sri Lanka. Proceedings of the 75th Annual Sessions of The Sri Lanka Association for the Advancement of Science; 79. Pusweldeniya, B. M. J. & Illeperuma, R. J. (2018) The success of cigarette and beedi butts as forensic DNA evidence in Sri Lanka. Annual Scientific Sessions of the Medico-Legal Society of Sri Lanka, Colombo, 2018. Nirmani M. D., Perera, K. L. N. & Galhena, G. H. (2017) The effect of extensive mosquito migration on dengue control as revealed by phylogeny of dengue vector Aedes aegypti. World Academy of Science, Engineering and Technology, International Science Index, Medical and Health Sciences, 4(12), 304. Available from: https://www.researchgate.net/publication/327305382_The_Effect_of_Extensive_Mosquito_Migration_on_Dengue_Control_as_Revealed_by_Phylogeny_of_Dengue_Vector_Aedes_aegypti Nirmani M. D, Perera, K. L. N. & Galhena, G. H. (2017) A comparison of genetic variability of dengue vectors using microsatellite and EPIC markers. Proceedings of Annual Research Symposium 2017, University of Colombo; 268. Available from: https://www.researchgate.net/publication/327305343_A_comparison_of_genetic_variability_of_dengue_vectors_using_microsatellite_and_EPIC_markers Nirmani M. D., Perera, K. L. N. & Galhena, G. H. (2017) Genetic diversity and population dynamics of dengue vectors Aedes aegypti and Aedes albopictus in selected localities in Sri Lanka. Proceedings of the 73rd Annual Sessions of The Sri Lanka Association for the Advancement of Science; 169. Available from: https://www.researchgate.net/publication/327305541_Genetic_diversity_and_population_dynamics_of_dengue_vectors_Aedes_aegypti_and_Aedes_albopictus_in_selected_localities_in_Sri_Lanka Tharaka, K. P. D. & Illeperuma, R. J. (2017) Amount of DNA in forensic hair evidence play minor role in success for conventional nuclear DNA testing, Annual Scientific Sessions of the Medico-Legal Society of Sri Lanka, Colombo, 2017. Nirmani, M. D., Gangani, P. D., Perera, K. L. N. & Galhena, G. H. (2016) Aedes aegypti population dynamics help evaluation of current vector control measures in Sri Lanka. Proceedings of Annual Research Symposium 2016, University of Colombo; 282. Available from: https://www.researchgate.net/publication/327305108_Aedes_aegypti_population_dynamics_help_evaluation_of_current_vector_control_measures_in_Sri_Lanka Gangani, P. D., Nirmani, M. D., Perera, K. L. N. & Galhena, G. H. (2016) Microsatellite markers reveal high genetic diversity among principal dengue vector, Aedes aegypti in Sri Lanka. Proceedings of the 36th Annual sessions of the Institute of Biology; 42. Nirmani, M. D., Gangani, P. D., Perera, K. L. N. & Galhena, G. H. (2016) Long distance passive migrations may explain the low genetic differentiation in principal dengue vector (Aedes aegypti) in Sri Lanka. Proceedings of the 36th Annual Sessions of the Institute of Biology; 43. Nirmani, M. D., Perera, K. L. N. & Galhena, G. H. (2015) Microsatellite markers reveal the spatial genetic structure of dengue vector Aedes aegypti in selected areas in Colombo district. Proceedings of the 35th Annual sessions of the Institute of Biology; 54. Available from: https://www.researchgate.net/publication/327305566_Microsatellite_markers_reveal_the_spatial_genetic_structure_of_dengue_vector_Aedes_aegypti_in_selected_areas_in_Colombo_district Nirmani, M. D., Madusanka, R. A., Perera, K. L. N. & Galhena, G. H. (2015) Use of ovitrap surveillance in assessing dengue outbreak risk in selected areas in Colombo district. Proceedings of Peradeniya University International Research Sessions 2015, University of Peradeniya; 229. Available from: https://www.researchgate.net/publication/327305370_Use_of_ovitrap_surveillance_in_assessing_dengue_outbreak_risk_in_selected_areas_in_Colombo_district Nirmani, M. D., Perera, K. L. N. & Galhena, G. H. (2015) Vector surveillance suggests an enhanced vector status for Ae. albopictus compared to Ae. aegypti in the Kandy district. Proceedings of Annual Research Symposium 2015, University of Colombo; 112. Available from: https://www.researchgate.net/publication/327305462_Vector_surveillance_suggests_an_enhanced_vector_status_for_Ae_albopictus_compared_to_Ae_argypti_in_Kandy_district Nirmani, M. D., Perera, K. L. N. & Galhena, G. H. (2015) Oviposition activity of dengue vectors in response to two different organic infusions. Proceedings of Annual Research Symposium 2015, University of Colombo; 113. Nirmani, M. D., Perera, K. L. N. & Galhena, G. H. (2015) Feasibility of using Exon-Primed Intron-Crossing (EPIC) markers to detect the genetic variation of a dengue vector in Sri Lanka. Proceedings of the 35th Annual Sessions of the Institute of Biology Sri Lanka; 60. Available from: https://www.researchgate.net/publication/327305637_The_feasibility_of_using_Exon-Primed_Intron-Crossing_EPIC_markers_to_detect_the_genetic_variation_of_a_dengue_vector_in_Sri_Lanka Kamaral, L. C. J., Perera, S. A. C. N., Perera, K. L. N. S. & Dasanayake, P. N. (2014). Population Structure of Sri Lanka yellow dwarf coconut variety as revealed by SSR markers. International Conference on Multidisciplinary Approaches 2014 (ICMA) pp 150. De Silva, W. H., Kamaral, L. C. J., Mohotti, S. N. K. & Perera, K. L. N. S. (2014) Development and validation of an allelic ladder for D18S51 short tandem repeat (STR) locus. Wayamba International Conference (WinC 2014). pp 251. Kamaral, L. C. J., Perera, K. L. N. S., Dasanayake, P. N. & Perera S. A. C. N. (2013) Variation of Sri Lanka yellow dwarf coconut variety at microsatellite marker loci. Proceedings of the 69th Annual Sessions. SLAAS. Part 1, pp 40. Gunasekera, J. M. A., Jayasekera, G. A. U., Fernandopulle, N. D., Perera, K. L. N. & De Silva, A. D. (2010) Development of Transgenic Plants for Leaffolder (Cnaphalocrosis medinalis) Resistance in Local Rice Varieties. Annual Research Proceedings, University of Colombo. pp 120. Available from: http://192.248.16.117:8080/research/handle/70130/381?mode=full 1 2

Testimonials “Taking part in “Advanced Certificate Course in Molecular Techniques for DNA Fingerprinting” and the “Internship Program in DNA Fingerprinting and Genetic Testing” have been a great experience for me. The hands-on training and detailed theoretical knowledge that I have gained from the course is invaluable, and the internship gave me a great understanding about real-world application of molecular biology techniques. The Scientists and instructors are really supportive and helped us to understand every single concept and all the practical applications from A to Z. I specially appreciate about providing an opportunity to carry out a research project, as I think that it would help me a lot by developing my research skills, scientific writing skills and a sense of confidence which will be really important in my further education and future career. I highly recommend this Course and the Internship to anyone who is passionate in the field of molecular biology and forensics.” Malisha Appuhami Post-A/L Student “I am excited to have completed the Advanced Certificate Course in Molecular Techniques for DNA fingerprinting at Genetech School of Gene Technology! As an ISO accredited laboratory, pioneering in molecular diagnostics, the facility instructed us on the use of various cutting edge molecular techniques focusing on, but not restricted to, DNA fingerprinting. Apart from this, students were provided with clear theory behind these techniques and DNA typing processes, including their applications and suitability across different cases. Despite the focus on forensics, it was clear these techniques are highly versatile and suitable for use across various aspects of life sciences. I am happy to share that I undertook the following as part of this course: Sample collection of biological evidence for DNA extraction DNA extraction using Chelex extraction methods and column-based DNA extraction kits DNA Quantification using the NanoDrop One spectrophotometer PCR amplification of STR markers and subsequent analysis via gel electrophoresis Multiplex PCR by AmpFLSTR™Identifiler™ PCR Amplification Kit for amplification of 15 STR loci Capillary electrophoresis using the ABI Genetic Analyzer 3500 and results interpretation via GeneMapper™ID-X Software Practical analysis of results/amplified STR markers for parentage testing Plant DNA extraction via CTAB method and amplification of the trnL intron barcoding region Mitochondrial sequence analysis using Chromas and MEGA software Sequence analysis for animal and plant barcoding using NCBI BLAST Grateful to the scientists and officers at Genetech for their guidance! Looking forward to using these skills soon!” Alina Marzook “A month has passed since I completed my three-month research project at Genetech and as I reflect on my time there, I’m overwhelmed with gratitude. This lab has shaped me in ways words can’t fully express & will always hold a special place in my heart. Walking into the lab every day was an incredible experience, not only professionally but also personally. I carry with me the knowledge and skills I gained, but more importantly, the memories made through challenges, breakthroughs, and those little triumphs that followed. This lab saw my moments of doubt transform into resilience. I’m deeply grateful to my supervisors and fellow interns for their guidance and inspiration, and I’m especially thankful to my Supervisor at Genetech Madam Mrs. Sumudu Wickramasekara. Her immense support and mentorship played a pivotal role in making this research a success. The camaraderie, encouragement, and support from everyone in the lab have truly made this journey unforgettable. Though our paths may diverge for now, I hope they cross again in the future. Thank you, Genetech for everything!” A student from SLIIT "The Advanced Certificate Course in Molecular Techniques for DNA Fingerprinting and the Internship Program were exceptional experiences that exceeded my expectations. The program offered abundant hands-on training, allowing me to refine my skills in DNA fingerprinting and genetic testing while gaining exposure to the latest advancements in molecular diagnostics and forensics. The supportive and approachable staff provided personalized guidance, ensuring a deep understanding of both foundational and advanced techniques. This practical, skills-focused approach made me industry-ready, and I was able to secure a job in the same field shortly after completing the program—a true testament to its quality and relevance. This course was worth far more than the investment, offering unparalleled opportunities to improve skills, expand knowledge, and gain a competitive edge in the job market. I highly recommend it to anyone looking to advance their career in molecular biology and diagnostics." Nimasha Liyanagedara, B.Sc (Hons), M.Sc. "The Advanced Certificate Course in Molecular Techniques for DNA Fingerprinting, as well as the Internship Program at Genetech, gave a unique chance to combine theory with practical experience. Working on a project focused on Y chromosome markers for forensic and kinship applications enhanced my skills in essential molecular techniques, including DNA extraction, PCR optimization, gel electrophoresis, and result analysis. Also, the internship helped me enhance my critical thinking and problem-solving skills as we worked on a research project. I am deeply grateful to the late Dr. Maya Gunasekera, founder of Genetech, whose legacy continues to inspire students like me. Also, I’d like to express my heartfelt gratitude to Dr. K.L. Nandika Perera, senior scientist and head of the department of molecular forensics at Genetech, as well as all the forensics scientists and Genetech staff, for their continuous encouragement, time, patience, and direction during our internship and research project. Thank you, Genetech, for everything. I wholeheartedly recommend this training to anyone seeking to advance in molecular genetics and forensic DNA analysis." Shashini Kariyawasam Undergraduate Student “I have successfully completed the internship at Genetech. This experience provided invaluable hands-on molecular laboratory practice, serving as a cornerstone in my academic and professional journey. A heartfelt thanks to madam Vajirapani de Silva, Ms. Yoshitha Wahalathanthri, Mrs. Sumudu Wickramasekara, Ms Bhagya Wijeratne, Mrs. Dimanthi Vithanage and Mr. Budhil de Silva for your unwavering guidance and support. Your mentorship has been invaluable.” Chamodi Jayathissa “I’m happy to share that I’ve obtained a new certification: Molecular Techniques for DNA Fingerprinting! Special thanks to Genetech and Dr Nandika for the amazing experience!” Methuli Linara Peththawadu

Discover Genetech’s journal publications on genetic research and advancements in Sri Lanka, shaping the future of molecular diagnostics and biodiversity. Sathkumara, H. D., Merritt, A. J., Corea, E. M., Krishnananthasivam, S., Natesan, M., Inglis, T. J. J. & De Silva, A. D. (2018) Clinical, Bacteriologic, and Geographic Stratification of Melioidosis Emerges from the Sri Lankan National Surveillance Program. Am J Trop Med Hyg . 98(2), 607-615. Available from: doi: 10.4269/ajtmh.17-0441 Grifoni, A., Weiskopf, D., Arlehamn, C. S. L., Angelo, M., Leary, S., Sidney, J., Frazier, A., Phillips, E., Mallal, S., Mack, S. J., Tippalagama, R., Goonewardana, S., Premawansa, S., Premawansa, G., Wijewickrama, A., De Silva, A. D. & Sette, A. (2018) Sequence-based HLA-A, B, C, DP, DQ, and DR typing of 714 adults from Colombo, Sri Lanka. Hum Immunol . 79(2), 87-88. Available from: doi: 10.1016/j.humimm.2017.12.007 Patil, V. S., Madrigal, A., Schmiedel, B. J., Clarke, J., O'Rourke, P., de Silva, A. D., Harris, E., Peters, B., Seumois, G., Weiskopf, D., Sette, A. & Vijayanand, P. (2018) Precursors of human CD4+ cytotoxic T lymphocytes identified by single-cell transcriptome analysis. Sci Immunol . 3(19). Available from: doi: 10.1126/sciimmunol.aan8664 Nanayakkara, K. K., Rodrigo, U. G., Perera, K. L. N. & Nanayakkara, C. D. (2017) Pre-natal diagnosis of thalassaemia in Sri Lanka: A ten year review. J Obstet Gynaecol . 37(7), 861-863. Available from: doi: 10.1080/01443615.2017.1306841 de Silva, W.S.I., Perera, M.M.N., Perera, K.L.N.S., Wickramasuriya, & A.M., Jayasekera G.A.U. (2017) In silico Analysis of osr40c1 Promoter Sequence Isolated from Indica Variety Pokkali. Rice Science, Vol. 24 (4): 228-234 Available from: doi: 10.1016/j.rsci.2016.11.002 . Gunasekara, J.M.A., Jayasekera, G.A.U, Perera, K.L.N.S. & Wickramasuriya, A.M, (2017) Development of a Sri Lankan rice variety Bg 94-1 harbouring cry2A gene of Bacillus thuringiensis resistant to rice leaffolder [Cnaphalocrocis medinalis (Guenée)]. Journal of the National Science Foundation of Sri Lanka, 45(2): 143–157. Available from: doi: 10.4038/jnsfsr.v45i2.8180 Goonawardhana, N.D.S., Jayasekara, G.S.K.W., Elanahai, V., Udagama, P.V., & Fernandopulle N.D. (2017) Population genetic data for ten miniSTR loci in the Sri Lankan population. International Journal of Legal Medicine. 131(4):969-970. Available from: doi: 10.1007/s00414-016-1512-3 Tian, Y., Babor, M., Lane, J., Schulten, V., Patil, V. S., Seumois, G., Rosales, S. L., Fu, Z., Picarda, G., Burel, J., Zapardiel-Gonzalo, J., Tennekoon, R. N., De Silva, A. D., Premawansa, S., Premawansa, G., Wijewickrama, A., Greenbaum, J. A., Vijayanand, P., Weiskopf, D., Sette, A. & Peters, B. (2017) Unique phenotypes and clonal expansions of human CD4 effector memory T cells re-expressing CD45RA. Nat Commun. 8(1), 1473. Available from: doi: 10.1038/s41467-017-01728-5 Grifoni, A., Angelo, M. A., Lopez, B., O'Rourke, P. H., Sidney, J., Cerpas, C., Balmaseda, A., Silveira. C. G. T., Maestri, A., Costa, P. R., Durbin, A. P., Diehl, S. A., Phillips, E., Mallal, S., De Silva, A. D., Nchinda, G., Nkenfou, C., Collins, M. H., de Silva, A. M., Lim, M. Q., Macary, P. A., Tatullo, F., Solomon, T., Satchidanandam, V., Desai, A., Ravi, V., Coloma, J., Turtle, L., Rivino, L., Kallas, E. G., Peters, B., Harris, E., Sette, A. & Weiskopf, D. (2017) Global Assessment of Dengue Virus-Specific CD4+ T Cell Responses in Dengue-Endemic Areas. Front Immunol. Available from: doi: 10.3389/fimmu.2017.01309 Grifoni, A., Pham, J., Sidney, J., O'Rourke, P. H., Paul, S., Peters, B., Martini, S. R., de Silva, A. D., Ricciardi, M. J., Magnani, D. M., Silveira, C. G. T., Maestri, A., Costa, P. R., de-Oliveira-Pinto, L. M., de Azeredo, E. L., Damasco, P. V., Phillips, E., Mallal, S., de Silva, A. M., Collins, M., Durbin, A., Diehl, S. A., Cerpas, C., Balmaseda, A., Kuan, G., Coloma, J., Harris, E., Crowe Jr, J. E., Stone, M., Norris, P. J., Busch, M., Vivanco-Cid, H., Cox, J., Graham, B. S., Ledgerwood, J. E., Turtle, L., Solomon, T., Kallas, E. G., Watkins, D. I., Weiskopf, D. & Sette, A. (2017) Prior Dengue virus exposure shapes T cell immunity to Zika virus in humans. J Virol. 91(24). Available from: doi: 10.1128/JVI.01469-17 Uehara, A., Tissera, H. A., Bodinayake, C. K., Amarasinghe, A., Nagahawatte, A., Tillekeratne, L. G., Cui, J., Reller, M. E., Palihawadana, P., Gunasena, S., De Silva, A. D., Wilder-Smith, A., Gubler, D. J., Woods, C. W. & Sessions, O. M. (2017) Analysis of Dengue Serotype 4 in Sri Lanka during the 2012-2013 Dengue Epidemic. Am J Trop Med Hyg. 97(1), 130-136. Available from: doi: 10.4269/ajtmh.16-0540 Krishnananthasivam, S., Jayathilaka, N., Sathkumara, H. D., Corea, E., Natesan, M. & De Silva, A. D. (2017) Host gene expression analysis in Sri Lankan melioidosis patients. PLoSNegl Trop Dis . 11(6). Available from: doi: 10.1371/journal.pntd.0005643 Krishnananthasivam, S., Sathkumara, H. D., Corea, E., Natesan, M. & De Silva, A. D. (2017) Gene Expression Profile of Human Cytokines in Response to Burkholderia pseudomallei Infection. mSphere . 2(2). Available from: doi: 10.1128/mSphere.00121-17 Grifoni, A., Angelo, M., Sidney, J., Paul, S., Peters, B., de Silva, A. D., Phillips, E., Mallal, S., Diehl, S. A., Botten, J., Boyson, J., Kirkpatrick, B. D., Whitehead, S. S., Durbin, A. P., Sette, A. & Weiskopf, D. (2017) Patterns of Cellular Immunity Associated with Experimental Infection with rDEN2Δ30 (Tonga/74) Support Its Suitability as a Human Dengue Virus Challenge Strain. J Virol . 91(8). 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M., Tan, S., Chow, A., Tummalapalli, P., De Silva, A. D., Østbye, T., Woods, C. W., Gubler, D. J. & Reller, M. E. (2016) Emergence of Epidemic Dengue-1 Virus in the Southern Province of Sri Lanka. PLoS Negl Trop Dis. 10(10). Available from: doi: 10.1371/journal.pntd.0004995 Kamaral, L. C. J., Dassanayaka, P. N., Perera, K. L. N. S. & Perera, S. A. C. N. (2016) SSR markers reveal the population structure of Sri Lankan yellow dwarf coconuts (Cocos nucifera L.). Tree Genetics & Genomes 12, 116. Available from: doi: 10.1007/s11295-016-1076-x Kirupananthan, R., Kamaral, L. C. J., Galhena, G. H., Perera, K. L. N. S. & Magamage, M. P. S. (2016) Address the public health and food security concerns of babesiosis through molecular detection of Babesia bovis in suspected carrier cattle of selected localities in Sri Lanka. Procedia Food Science 6: 213-219. Available from: doi: 10.1016/j.profoo.2016.02.053 Weiskopf, D., Angelo, M. A., Grifoni, A., O'Rourke, P. H., Sidney, J., Paul, S., De Silva, A. D., Phillips, E., Mallal, S., Premawansa, S., Premawansa, G., Wijewickrama, A., Peters, B. & Sette, A. (2016) HLA-DRB1 Alleles Are Associated With Different Magnitudes of Dengue Virus-Specific CD4+ T-Cell Responses. J Infect Dis. 214(7), 1117-1124. Available from: doi: 10.1093/infdis/jiw309 de Alwis, R., Bangs, D. J., Angelo, M. A., Cerpas, C., Fernando, A., Sidney, J., Peters, B., Gresh, L., Balmaseda, A., de Silva, A. D., Harris, E., Sette, A. & Weiskopf, D. (2016) Immunodominant Dengue Virus-Specific CD8+ T Cell Responses Are Associated with a Memory PD-1+ Phenotype. J Virol. 90(9), 4771-4779. Available from: doi: 10.1128/JVI.02892-15 Olsen, A., Chen, Y., Ji, Q., Zhu, G., De Silva, A. D., Vilchèze, C., Weisbrod, T., Li, W., Xu, J., Larsen, M., Zhang, J., Porcelli, S. A., Jacobs Jr, W. R. & Chan, J. (2016) Targeting Mycobacterium tuberculosis Tumor Necrosis Factor Alpha-Downregulating Genes for the Development of Antituberculous Vaccines. MBio. 7(3). Available from: doi: 10.1128/mBio.01023-15 Tissera, H., Amarasinghe, A., Gunasena, S., De Silva, A. D., Yee, L. W., Sessions, O., Muthukuda, C., Palihawadana, P., Lohr, W., Byass, P., Gubler, D. J. & Wilder-Smith, A. (2016) Laboratory-Enhanced Dengue Sentinel Surveillance in Colombo District, Sri Lanka: 2012-2014. PLoS Negl Trop Dis. 10(2). Available from: doi: 10.1371/journal.pntd.0004477 Weiskopf, D., Angelo, M., Zapardiel, J., Seumois, G., De Silva, A., De Silva, A. D., Vijay, P., Peters, B. & Sette, A. (2016) DENV-specific CD4 T-cells dominantly recognize capsid-derived epitopes and display a cytotoxic phenotype. J Immunol . 196 (1). Available from: https://www.jimmunol.org/content/196/1_Supplement/147.13/tab-article-info Padumadasa, C., Perera, K. L. N. & Sankalpani, E. M. N. (2016) DNA intercalation and cleavage studies of plumbagin and phenanthroline-based Cu(II) complex, [Cu(PLN)(PHEN)]NO3. Int J Chem Stud. 4(4), 87-90. Available from: https://www.chemijournal.com/archives/?year=2016&vol=4&issue=4&ArticleId=282&si=false Fernando, A. N., Malavige, G. N., Perera, K. L. N., Premawansa, S., Ogg, G. S. & De Silva, A. D. (2015) Polymorphisms of Transporter Associated with Antigen Presentation, Tumor Necrosis Factor-α and Interleukin-10 and their Implications for Protection and Susceptibility to Severe Forms of Dengue Fever in Patients in Sri Lanka. Journal of Global Infectious Diseases. 7(4): 157–164. Available from: doi: 10.4103/0974-777X.170501 Weiskopf, D., Cerpas, C., Angelo, M. A., Bangs, D. J., Sidney, J., Paul, S., Peters, B., Sanches, F. P., Silvera, C. G., Costa, P. R., Kallas, E. G., Gresh, L., de Silva, A. D., Balmaseda, A., Harris, E. & Sette, A. (2015) The Human CD8+ T-Cell Responses Against the 4 Dengue Virus Serotypes Are Associated With Distinct Patterns of Protein Targets. J Infect Dis . 212(11), 1743-1751. Available from: doi: 10.1093/infdis/jiv289 Page 2 of 3 Journal Publications 1 2 3

Explore Genetech’s commitment to innovation in biotechnology at our Research Institute (GRI). Pioneering not-for-profit research in Sri Lanka, we welcome diverse minds to shape the future with us. Improvement with innovation Research is the foundation of all our activities. Whilst most of the methodologies we adopt have been researched and developed by us, research is constantly being conducted to optimise and improve our methods. Not-for-profit research We have a not-for-profit research division known as Genetech Research Institute (GRI), which is managed by a Board of Trustees headed by Dhammika Gunasekera. Learn more Active involvement locally and globally GRI is the pioneer not-for-profit private scientific research facility in Sri Lanka, and has in its short history been able to receive research grants from national and international granting bodies. We also actively take part in scientific sessions and symposia locally and internationally. Our welcoming culture Our research facility welcomes independent researchers, undergraduate and graduate research students as we believe diversity is important in creating future-ready solutions using cutting-edge technology.

Explore innovative PhD projects funded by Genetech in Sri Lanka, covering forensic genetics, dengue vector studies, and rice blast resistance. Dive into cutting-edge research and groundbreaking discoveries! Research leading to the award of PhD X-Chromosome markers for forensic genetics in Sri Lanka: development, validation and application of a novel 16 X-STR multiplex assay for the Sri Lankans. K. L. Nandika Perera, Ph.D., Department of Basic Sciences, Faculty of Health Sciences, The Open University of Sri Lanka, 2021. Population genetic and epidemiological investigation of the dengue vectors Aedes aegypti (L.) and Aedes albopictus (Skuse) in Sri Lanka. M. D. Nirmani, Ph.D., Department of Zoology and Environment Sciences, Faculty of Science, University of Colombo, Sri Lanka, 2021 (Thesis Submitted). DNA marker assisted breeding of rice blast resistant genes into local rice. Ama Jayawardana, Ph.D. Department of Plant Sciences, Faculty of Science, University of Colombo; Rice Research and development Institute, Batalagoda, 2019 (Conducted partially at Genetech). Genetic diversity and population structure of ex-situ conserved finger millet (Eleusine coracana (L.) Gaertn) accessions in Sri Lanka. Parami Wakista, Ph.D. Department of Botany, Faculty of Applied Science, University of Sri Jayewardenepura, 2017. Development of Mini STR primers in order to enhance sensitivity and amplification efficiency of Polymerase Chain Reaction (PCR) to aid analysis of degraded DNA evidence in Sri Lankan case work. N. D. Suraj Goonawardhana, Ph.D., Department of Zoology Environment Sciences, Faculty of Science, University of Colombo, Sri Lanka, 2016. Characterization and transfer of Drought Responsive Elements Binding (DREB) genes to develop transgenic drought resistant rice. W.S. Imesh De Silva, Ph.D., Department of Plant Sciences, Faculty of Science, Faculty of Science, University of Colombo, Sri Lanka, 2016. Understanding the role of host genetic factors and specific dengue virus epitomes during dengue infection. Anira Fernando, Ph.D., Department of Zoology Environment Sciences, Faculty of Science, University of Colombo, Sri Lanka, 2015. Sustainable insect resistance in local rice varieties. Manisha Gunasekera, PhD, Department of Plant Sciences, University of Colombo, Sri Lanka. 2010 (partially conducted at Genetech). Development of databases for autosomal Y-chromosomal and mitochondrial DNA markers and their application in forensic casework and population genetics in Sri Lankan populations. Ruwan J Illeperuma, PhD, Department of Zoology, University of Colombo, Sri Lanka, 2010. Detection, quantification and characterization of Hepatitis C virus strains in Sri Lanka. Dhammika B. Seneviratne, PhD, Postgraduate Institute of Science, University of Peradeniya, Sri Lanka, 2010. Severity of dengue disease and the role of different serotypes and genotypes of the causative viruses. Upul Nalaka De Silva Kanakaratne, PhD, Department of Microbiology, Faculty of Medicine, University of Peradeniya, Sri Lanka, 2009.

Discover GRI’s commitment to high ethical and legal standards in scientific research. Learn about our rigorous guidelines for human subjects, bio-hazardous agents, and international compliance. Legal & Ethical Guidelines GRI will conform with all existing Laws of Sri Lanka regarding the performance of scientific research. Where such Laws do not exist, we will seek guidance from regional and international Laws. GRI will act as a responsible scientific entity, and will maintain high ethical and legal standards at every stage of the research project. We will require the following from all external researchers: All projects involving human subjects or human specimens will be evaluated by a GRI institutional review board (IRB). Human subject research requires the approval of the GRI IRB as well as the IRB of the investigators' home institution. A written guarantee of the source, the collection methodology of the tested material and the end use of the data is required. For samples with restricted access such as protected or endangered species, the external researcher has to provide copies of permits or permission from relevant authorities. GRI retains the right to verify the legal and ethical status of any tested material, in consultation with relevant authorities or experts. GRI retains the right to terminate the research project at any time if it is discovered that that the tested material was obtained illegally or unethically. GRI will not be held responsible for any observations and conclusions made from such research, or for any subsequent action taken by any person in response to such observations and conclusions (except when the research is performed in collaboration with a senior scientist at GRI). Projects involving work with bio-hazardous agents will be reviewed and only undertaken if the specimens do not pose a significant risk to people working on the project or others. Only international shipments that are compliant with local and foreign import/export regulations will be allowed.

Join our 60-hour Intensive Practical Course in Molecular Biological Techniques at Genetech. Gain hands-on experience in cloning, DNA/RNA extraction, PCR, and gel electrophoresis. Ideal for researchers, students, and professionals in biological, medical, and forensic sciences. Intensive Practical Course in Molecular Biological Techniques (60 hours) This intensive training course provides a thorough hands on experience in key molecular biological techniques used in various fields of biological, medical and forensic sciences. The course is primarily aimed at individuals who are new to the area, theoretically familiar but lacking in practical bench training or wishing to enhance their practical skills further. At the end of the course, the participants will be able to acquire an adequate level of proficiency to allow for the troubleshooting and optimisation of routine laboratory protocols to promote timeliness and cost effectiveness. All practical work is conducted individually with the guidance of experienced persons in the relevant field. When? This course will run from 9.00am to 4.00pm for 10 consecutive weekdays as a full-time programme. Course dates: TBD For whom? Senior and junior researchers who wish to establish in the field of molecular biology Post A/L students interested in pursuing future studies in biological/biomedical sciences and/or forensic sciences Individuals who seek career opportunities in medical diagnostic laboratories and forensic DNA laboratories Those who wish to complement their knowledge in modern molecular biological techniques (e.g., medical doctors, medical laboratory technicians, forensic analysts, industry persons) Techniques Covered Cloning techniques: Isolation of plasmid DNA, Restriction enzyme digestion and transformations DNA extraction techniques: Isolation of human genomic DNA from blood and forensic samples, Bacterial/Viral DNA and Plant DNA RNA work: Isolation/purification and reverse transcriptase PCR assays Polymerase Chain Reaction: Technical elements and critical factors for successful PCR-based procedures, Multiplex PCR, Real Time PCR and PCR with RFLP Gel Electrophoresis: Agarose and polyacrylamide gel electrophoresis, automated capillary electrophoresis Course Fee Rs. 75,000 per person View FAQs Contact us If you are interested in doing this course at Genetech, please email us at info@genetechsrilanka.com to receive further details.

Explore our comprehensive molecular biology internships, including disease diagnostics, DNA fingerprinting, and GM food testing. Hands-on training, expert supervision, and individual research assignments. Internship Programme on Molecular Disease Diagnostics Who Can Register: A/L, Pre-University and Undergraduate Students Our internship programme offers the flexibility to choose between two-week and six-week durations, tailored to your educational needs. Available throughout the year, this programme is designed to provide hands-on experience in molecular diagnostics, with the ability to customise based on specific requirements. Two-Week Programme: LKR 64,500 Key Focus Areas: Diagnostic Testing: Human infectious disease testing Cancer testing Canine disease testing Plant disease testing Methods covered: DNA extraction/handling of different samples RNA extraction PCR, RT-PCR, qPCR Gel electrophoresis PCR trouble shooting Introduction to the ISO 15189 system and planning a molecular diagnostic laboratory Six-Week Programme: LKR 120,000 Includes all components of the two-week programme. In addition, interns will undertake a four-week internship involving an individual research assignment. This project involves a literature survey, independent laboratory work, result analysis, and interpretation. Upon successful completion of the programme, interns will receive a certification letter detailing their training and experience. View FAQs Contact us If you are interested in doing an internship at Genetech, please submit the Inquiry Form below or email us at info@genetechsrilanka.com to receive further details. View Inquiry Form