GENETECH 

Untitled.png

COVID-19 Alert!

Due to the prevailing situation please schedule an appointment by contacting us via phone/email prior to visiting GENETECH.

Untitled.png
  • HOME

  • About Us

  • Diagnostics

  • Training

  • GRI

  • Bio Stores

  • Contact Us

  • More...

    Course 4: Polymerase Chain Reaction (PCR) Technology (14 hours)

     

    This course is suitable for school-level, undergraduate and graduate students, and those engaged in scientific research, diagnostics and teaching which involves Polymerase Chain Reaction (PCR) Technology. 

     

     

    Theory:

     

    • Guidelines for PCR

    • Principle of PCR

    • Critical factors for successful PCR

    • Designing PCR primers

    • Standard practices in a PCR Laboratory

    • Troubleshooting and prevention of carryover contamination

    • Optimization strategies for PCR

     

         PCR product analysis:

     

    • Detection of PCR products by gel electrophoresis

    • Methods of purification and quantification of PCR products

    • Methods of labelling of PCR products (PCR probes) and visualization of label

    • Cloning of PCR products: TOPO cloning

    • DNA sequence analysis of PCR product 

     

         PCR Applications:

     

    • Restriction Fragment Length Polymorphism (RFLP) and Randomly Amplified Polymorphic DNA (RAPD) analysis

    • Short Tandem Repeat (STR) (micro-satellite) analysis

    • Multiplex PCR

    • Reverse Transcription (RT)-PCR

    • PCR-ELISA (Enzyme Linked Immunosorbent Assay)

    • In-situ PCR

    • Quality Control/Quality Assurance of PCR assays

     

     

    Practicals:

     

    • Extraction of DNA from cells, blood stains for PCR

    • A standard PCR assay

    • Analysis of PCR products by agarose and acrylamide gel electrophoresis

    • Strategies for optimization of PCR

    • Purification of PCR products

    • Labelling of PCR products

    • Primer design using online sequence databases

    • PCR-RFLP assay

    • Multiplex PCR assay

    • RT-PCR assay

    • PCR-ELISA assay

    • PCR troubleshooting

    ​

    ​

    ​

    ​

    ​

    ​

    ​