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    Course 2: Certificate Course in Gene Technology

    (60 hours)

     

    Theory:

     

    •    DNA, RNA structure and function

    •    Messenger RNA, Transfer RNA & proteins

    •    Chromosomes, genes, alleles, karyotypes, phenotype & genotype

    •    Extracting DNA from different sources

    •    Basic methods in bacteriology

    •    DNA modifying enzymes

    •    Genetic engineering & gene splicing

    •    Electrophoresis & quantification of DNA

    •    Polymerase chain reaction

    •    DNA fingerprinting in forensic casework

    •    Biotechnology in medicine & industry

     

    Practicals:

     

    (Hands-on practical experience on an individual basis for each student)

     

    •    Laboratory rules & safety precautions

    •    Use of micropipettes

    •    Preparation of reagents

    •    Plating & quantifying of bacteria

    •    Forensic DNA testing: Extraction of DNA, PCR & gel electrophoresis

    •    Isolation of plasmid DNA

    •    Spectrophotometric determination of DNA

    •    DNA sequence data analysis

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