Course 2: Certificate Course in Gene Technology

(60 hours)

 

Theory:

 

•    DNA, RNA structure and function

•    Messenger RNA, Transfer RNA & proteins

•    Chromosomes, genes, alleles, karyotypes, phenotype & genotype

•    Extracting DNA from different sources

•    Basic methods in bacteriology

•    DNA modifying enzymes

•    Genetic engineering & gene splicing

•    Electrophoresis & quantification of DNA

•    Polymerase chain reaction

•    DNA fingerprinting in forensic casework

•    Biotechnology in medicine & industry

 

Practicals:

 

(Hands-on practical experience on an individual basis for each student)

 

•    Laboratory rules & safety precautions

•    Use of micropipettes

•    Preparation of reagents

•    Plating & quantifying of bacteria

•    Forensic DNA testing: Extraction of DNA, PCR & gel electrophoresis

•    Isolation of plasmid DNA

•    Spectrophotometric determination of DNA

•    DNA sequence data analysis