Course 2: Certificate Course in Gene Technology
(60 hours)
Theory:
• DNA, RNA structure and function
• Messenger RNA, Transfer RNA & proteins
• Chromosomes, genes, alleles, karyotypes, phenotype & genotype
• Extracting DNA from different sources
• Basic methods in bacteriology
• DNA modifying enzymes
• Genetic engineering & gene splicing
• Electrophoresis & quantification of DNA
• Polymerase chain reaction
• DNA fingerprinting in forensic casework
• Biotechnology in medicine & industry
Practicals:
(Hands-on practical experience on an individual basis for each student)
• Laboratory rules & safety precautions
• Use of micropipettes
• Preparation of reagents
• Plating & quantifying of bacteria
• Forensic DNA testing: Extraction of DNA, PCR & gel electrophoresis
• Isolation of plasmid DNA
• Spectrophotometric determination of DNA
• DNA sequence data analysis