Theory:

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• DNA, RNA structure and function

• Messenger RNA, Transfer RNA & proteins

• Chromosomes, genes, alleles, karyotypes, phenotype & genotype

• Extracting DNA from different sources

• Basic methods in bacteriology

• DNA modifying enzymes

• Genetic engineering & gene splicing

• Electrophoresis & quantification of DNA

• Polymerase chain reaction

• DNA fingerprinting in forensic casework

• Biotechnology in medicine & industry

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Practicals (hands-on practical experience on an individual basis for each student):

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• Laboratory rules & safety precautions

• Use of micropipettes

• Preparation of reagents

• Plating & quantifying of bacteria

• Forensic DNA testing: Extraction of DNA, PCR & gel electrophoresis

• Isolation of plasmid DNA

• Spectrophotometric determination of DNA

• DNA sequence data analysis