Course 1: Advanced Certificate Course in Gene Technology (80 hours)

 

Theory:

 

  • Introduction to Gene Technology

Basic unit of life, transfer of genetic material, DNA, genomic DNA, the gene, allele, genotype, phenotype, different types of genes, RNA, information flow, applications of molecular biology and gene technology, structure, chemistry and physical properties of nucleotides, organization of DNA in the cell.

 

  • Gene Expression and Proteins

Central dogma of life, gene expression, genetic code, degeneracy of the genetic code, amino acids, reading frame, mutations, DNA repair, DNA replication, transcription of DNA, translation of RNA, proteins and proteomics, transcriptional regulation.

 

  • Recombinant DNA Technology

 

  • Tools in DNA Analysis

  1. Extracting DNA from different sources: introduction to DNA extraction, steps in extraction, extracting DNA from forensic evidentiary samples.

  2. Forensic DNA typing: DNA typing, DNA based human identification, identification of biological evidence, paternity testing, etc.

  3. Polymerase Chain Reaction (PCR)

  4. Gel electrophoresis and Spectrophotometry

 

  • Micro RNA and Cancer Detection

 

  • Transgenic Technology

Development of transgenic animals and plants

 

  • Immunology

 

  • The Human Genome Project
     

  • Current Applications and Ethical Aspects of Gene Technology

 

Practicals:

 

(Hands-on practical experience on an individual basis for each student)

 

•    Laboratory safety

•    Preparation of  molecular biology solutions and accessories

•    Extraction of genomic DNA, quantification, and assessment of the quality of DNA

•    Culture and transformation of E.coli

•    Isolation of plasmid DNA from E. coli

•    Preparation of agarose and polyacrylamide gels

•    Analysis of native and restricted plasmid and genomic DNA by agarose gel electrophoresis

•    Analysis of PCR products using polyacrylamide gel electrophoresis

•    DNA typing of simulated crime scene sample 

•    Southern transfer and hybridization of DNA

•    DNA library screening and hybridization

•    Standard PCR assay

•    Multiplex PCR

•    RT-PCR assay for viral DNA detection 

•    Primer designing with software

•    DNA sequence data analysis

•    Genetic database searching